High-throughput mouse phenomics for characterizing mammalian gene function
نویسندگان
چکیده
منابع مشابه
Computational approaches to phenotyping: high-throughput phenomics.
The recent completion of the Human Genome Project has made possible a high-throughput "systems approach" for accelerating the elucidation of molecular underpinnings of human diseases, and subsequent derivation of molecular-based strategies to more effectively prevent, diagnose, and treat these diseases. Although altered phenotypes are among the most reliable manifestations of altered gene funct...
متن کاملStatistical methods for comparative phenomics using high-throughput phenotype microarrays.
We propose statistical methods for comparing phenomics data generated by the Biolog Phenotype Microarray (PM) platform for high-throughput phenotyping. Instead of the routinely used visual inspection of data with no sound inferential basis, we develop two approaches. The first approach is based on quantifying the distance between mean or median curves from two treatments and then applying a per...
متن کاملCharacterizing Transcriptomes from High-Throughput Sequencing Data
The DNA in our cells contains our genetic hereditary information, and is literally the blueprint of our body. The functional units of the genome are regions of continuous DNA sequence, and are called genes. According to the Central Dogma of Biology, the DNA sequence of the gene is transcribed into a messenger RNA (mRNA), which is in turn translated to proteins, which perform most tasks in the c...
متن کاملLarge-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos
We have adapted the whole-mount in situ hybridization technique to perform high-throughput gene expression analysis in mouse embryos. A large-scale screen for genes showing specific expression patterns in the mid-gestation embryo was carried out, and a large number of genes controlling development were isolated. From 35760 clones of a 9.5 d.p.c. cDNA library, a total of 5348 cDNAs, enriched for...
متن کاملMouse mutagenesis-systematic studies of mammalian gene function.
The mouse will play a pivotal role in mammalian gene function studies as we enter the post-genomics era. The challenge is to develop systematic, genome-wide mutagenesis approaches to the study of gene function. The current mouse mutant resource has been an important source of human genetic disease models. However, despite an apparently large catalogue of mouse mutations, we have access to mutat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Reviews Genetics
سال: 2018
ISSN: 1471-0056,1471-0064
DOI: 10.1038/s41576-018-0005-2